Device

Part:BBa_K4407010:Design

Designed by: Leyu Xu   Group: iGEM22_Worldshaper-Nanjing   (2022-10-05)


Pthl-dps，Expression of dps with Pthl promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promotor Pthl starts the transcription of dps gene and the terminator ends the transcription once it is completed. dps protein protects the bacteria from oxidative damage. It protects DNA against oxidative damage by binding with Fe2+ to prevent it from reacting with H2O2 (Fenton’s reaction). In our project, we used this device to enhance the viability of C. tyrobutyricum in aerobic environment.


Source

Pthl is based on BBa_K3443002. RBS is based on part BBa_K103015. dps is based on BBa_K4407001. Terminator is based on BBa_K3585002.

References